现在，艾吉奥与妻子和两个孩子住在托斯卡纳一座舒适的别墅里，他把时间都花在了与家人、老朋友的相聚和葡萄园的耕耘上。但这位前刺客导师的生活不会平静太久。一天，一个神秘的刺客突然出现在艾吉奥的家门口，恳求艾吉奥的帮助。这位老刺客的火焰是在拯救他所爱的人之前熄灭，还是最后一次熊熊燃烧？

In the year 1524, the former Assassin Ezio Auditore retired to a villa in the countryside of Tuscany, with his wife Sofia and children Marcello and Flavia. While Ezio wrote in his study, Sofia asked him to watch Flavia while she and Marcello visit his sister Claudia. Shortly after, he left his study to tend their viney…